Chris Basham says United will be ready to go again after a hard-working effort at Villa Park failed to bring points following a narrow 1-0 defeat.
The Blades worked tirelessly in difficult circumstances after playing 78 minutes with 10 men following John Egan's red card, but Ezri Konsa's second-half header secured maximum points for the hosts.
Basham said he thought United reacted well to the setback, and still fancied the task in hand, despite the numerical disadvantage.
United, who could have gone in front but for John Lundstram's saved penalty, kept going to the end and Basham believes that's the attitude they'll take going forward as they look to quickly bounce back at the weekend.
But there are plenty of positives to take.
The Blades, down to 10-men after 12 minutes, were always in a tight contest despite the numerical disadvantage, and could have gone in front after John Lundstram's penalty was saved, before Ezri Konsa's header won the game for Villa.
Basham outlined several positives, which included the form of new signings Oli Burke and Ethan Amapdu, as competition for places intensifies at Bramall Lane.
The versatile defender says United will be up for the battle once again as attention turns to Sunday's showdown with Leeds at Bramall Lane.