Dan O'Hearne has stepped down from his position as first team manager with United Ladies with immediate effect.
General manager Lee Walshaw: "On behalf of everybody at Sheffield United, I'd like to thank Dan for his hard work, commitment and the success he's brought the club over the last four years. The club is certainly in a better place now than when he arrived. We would have liked Dan to remain with us and it's sad to see him go but it's his decision and one we must accept and respect."
Dan, 45, became United's manager at the start of the 2014/15 season, his first role in women's football and last season was instrumental as United Ladies won the East Midlands Premier League.
Carla Ward will be interim manager until further notice.