Blades 0-0 Leicester - Leicester win pens
United began the Checkatrade Trophy campaign with a point, but missed out on a second point losing 5-4 on penalties to Leicetser City after the game finished goalless after 90 minutes.
The Premier League champions, who included a handful of first-team players scored all their penalties, with John Fleck's miss proving the difference between the two sides following an evenly fought contest.
Boss Wilder made four changes for the clash against the Foxes, with Chapman handed a first start. Fleck, O'Connell and Reed were also named in the starting XI, as Wright, Basham and Duffy dropped to the bench. Scougall missed out with an injury sustained at the weekend.
United made a bright start and Fleck's inswinging corner from the right was inches too high for a number of onrushing red and white shirts. Then in the 9th minute, Sharp prodded inches wide with the goal at his mercy after good work by Done. At the other end, Benalouane headed over from Hernandez's long throw.
Midway through the first-half, the visitors made a change with goalkeeper Hamer being replaced by Bramley. As the clocked ticked past the half hour there was little to report in the way of goalmouth action, although Fleck had Bramley scrambling across his goal from a long-range free-kick.
With the clock ticking down for the interval, Rowe fired into the side netting for the Foxes, whilst Hussey sent a rasping drive over the bar on the run for the Blades.
Both teams made a change at the break, Duffy replaced Sharp for United, whilst Moore took the place of Benalouane at the heart of the visitors defence.
In 50th minute, Moore was at his best to tip over Gray's deflected effort. For the Blades, Chapman got the better of Hernandez before stinging the palms. United's number 32, then came even closer to opening the scoring curling just wide after Done had rushed Bramley into a clearance.
Just after the hour, Brooks was introduced for his senior debut, replacing Done and the youngster's impact was almost immediate as he played in Reed, but the midfielder failed to take the ball cleaning under pressure from Wasilewski. Moments later, Brooks was booked for a late challenge on the advancing Gray.
The Blades were gaining the upper hand and only a good save from Bramley, who stood tall, denied the advancing Chapman who had broken City's offside trap. Brooks was looking a live threat and again, only a save from Bramley saved City.
With less than five minutes remaining, City came close to snatching the winner, but Moore denied Moore, as Simon tipped over from Elliott's header.
Two minutes of injury-time were signalled as Duffy came close to breaking the deadlock only to see his shot deflected agonisingly wide.
That was the last action of the 90 minutes, as the game went to penalties to see who claimed the second point on the night.
Coutts scored (1-0)
Chilwell scored (1-1)
Fleck saved (1-1)
Gray scored (1-2)
Duffy scored (2-2)
Muskwe scored (2-3)
Chapman scored (3-3)
Barnes scored (3-4)
O'Connell scored (4-4)
Ndukwu scored (4-5)
Blades: Moore, Hussey, Fleck, O'Connell, Sharp (Duffy 45), Done (Brooks 63), Coutts, Freeman, Wilson, Reed, Chapman.
Unused: Long, Basham, Wright, K. Wallace, Whiteman.
Leicester: Hamer (Bramley 24), Hernandez, Chilwell, James (Barnes 76), Gray, Wasilewski, Benalouane (Moore 45), Rowe, Miles, Muskwe, Ndukwu.
Unused: Barnes, Mitchell, Wood, Watts, Pascanu.