The match-worn shirts from Sheffield United's game against Port Vale will be auctioned for charity in memory of Blades fan David "Shred" Spencer.
The game gave fans the opportunity to see next season's strip for the first time as the Blades ran out wearing the new Adidas-designed kit.
The shirts will now go for sale on eBay, with all profits going to the British Heart Foundation (BHF), the charity that Shred's family supported.
All shirts are embroidered with the date of the game, opponents and the British Heart Foundation logo, just above the Blades badge. Fans can bid for one of these unique shirts, from 3pm on Friday 27th March, by visiting www.ebay.co.uk/sch/sufc_official/m.html?_ipg=50&_sop=12&_rdc=1
Dave McCarthy, Operations Director of Sheffield United, said: "We hope that by auctioning these unique shirts worn by the players at the weekend, that we can go some way to raising money for a worthy cause and one that is close to the Club's heart."
Meanwhile, fans wanting to order the new 2015/16 home strip can pre-order online, with all pre-orders dispatched the week commencing 27th April, ahead of general sale.